Product description
Contents
Taq Plus DNA Polymerase (2.5 U/μl): 100 μl
10xPCR Buffer (Mg2+ Plus): 1.25 ml
dNTPs (each 2.5 mM): 1 ml
6xLoading Buffer: 1 ml
Note 10×PCR Buffer (Mg2+ Plus) can replace with 10×PCR Buffer (Mg2+ free) and 25 mM MgCl2. Please choose the appropriate package for your experiment. Description Taq Plus, a mixture of taq and pfu polymerase, blends the processivity of taq with the high fidelity of pfu. Therefore, this specially formulated Taq plus allow amplification of the higer fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3´-dA overhangs PCR products, which can be used in TA clone. Features High fidelity: Its fidelity is four times higher than ordinary Taq polymerase. High yields: Suitable for amplifying large fragment, suitable for complex template which is rich in GC or repeat sequence. Applications Long PCR with high fidelity Amplification reaction of complex template Quality Control The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
Functionally tested in PCR Definition of Activity Unit One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate. Storage Buffer
20mM TrisCl ( pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT，0.1% NP-40 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol
10X PCR Buffer
100mM Tris-HCl(PH 8.8), 500mMKCl, 1%Triton-X-100, 16mM MgCl2
Store all components at –20°C