Product description
Rnase inhibitor: 1000U Concentration: 40 U/μl Store at -20°C Description: RNasin is a ribonuclease inhibitor purified from human placenta. RNasin reduces the activity of RNase A-type enzymes in a variety of organisms. Applications:
Inhibition of RNA degradation in the following procedures:
-in vitro transcription -cDNA synthesis -in vitro translation
isolation of mammalian cell fractions that contain mRNA-protein complex；
Separation and identification of specific ribonuclease activities；
Studies of tumor suppression；
Quality Control: The absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.
Functionally tested in RNA and cDNA synthesis. Definition of Activity Unit: One unit of the protein inhibits the activity of 5ng RNase A by 50%. Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml E.coli [3H]-RNA, 50 mg/ml yeast RNA, 8 mM DTT.