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MEDICO 

A Company In Developing Life Sciences Products for Research

COPYRIGHT© 2017 by Medico. All rights reserved.

Product description

 

Rnase inhibitor: 1000U 
Concentration: 40 U/μl

Store at -20°C

Description:
RNasin  is a ribonuclease inhibitor purified from human placenta. RNasin  reduces the activity of RNase A-type enzymes in a variety of organisms.

Applications:

  • Inhibition of RNA degradation in the following procedures:

-in vitro transcription
-cDNA synthesis
-in vitro translation

  • isolation of mammalian cell fractions that contain mRNA-protein complex;
  • Separation and identification of specific ribonuclease activities;
  • Studies of tumor suppression;


Quality Control:
The  absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases  and ribonucleases confirmed by appropriate quality tests.

Functionally tested in RNA and cDNA synthesis.

Definition of Activity Unit:
One unit of the protein inhibits the activity of 5ng RNase A by 50%.
Inhibitor  activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5),  1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml E.coli  [3H]-RNA, 50 mg/ml yeast RNA, 8 mM DTT.

RNase inhibitor

SKU: MD-RNI-01
$67Price
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