Product name

Rat IL-25 (Interleukin 25) ELISA Kit

Catalogue No.

ER1097

Detection method

Sandwich ELISA, Double Antibody

Application

IL-25 ELISA Kit allows for the in vitro quantitative determination of IL-25 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-25 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 92-98 95
EDTA plasma(n=5) 87-102 96
heparin plasma(n=5) 85-103 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-104% 95-104% 86-91% 86-98%
EDTA plasma(n=5) 83-94% 85-95% 82-94% 86-96%
heparin plasma(n=5) 81-99% 84-100% 93-99% 83-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Rat IL-25 (Interleukin 25) ELISA Kit

SKU: ER1097
$360Price
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