Product name | Rat IL-1β (Interleukin 1 Beta) ELISA Kit |
Catalogue No. | ER1094 |
Detection method | Sandwich ELISA, Double Antibody |
Application | IL-1β ELISA Kit allows for the in vitro quantitative determination of IL-1β concentrations in plasma, tissue homogenates and other biological fluids. |
Size | 96T |
Range | 31.25-2000pg/ml |
Sensitivity | < 18.75pg/ml |
Storage | 4 ℃ for 6 months |
Recovery | Matrices listed below were spiked with certain level of IL-1β and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β in samples. |
Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. |
CV(%) | Intra-Assay: CV<8% Inter-Assay: CV<10% |
Note | For Research Use Only |
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-105 | 95 |
EDTA plasma(n=5) | 87-99 | 93 |
heparin plasma(n=5) | 86-104 | 95 |
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 85-101% | 86-96% | 85-105% | 88-105% |
EDTA plasma(n=5) | 88-98% | 83-99% | 85-100% | 86-101% |
heparin plasma(n=5) | 83-92% | 84-99% | 84-95% | 87-97% |