Product name
Rat IL-1α (Interleukin 1 Alpha) ELISA Kit
Catalogue No.
ER1093
Detection method
Sandwich ELISA, Double Antibody
Application
IL-1α ELISA Kit allows for the in vitro quantitative determination of IL-1α concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1α and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1α in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
96
EDTA plasma(n=5)
88-100
97
heparin plasma(n=5)
85-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
88-99%
88-103%
88-103%
89-102%
EDTA plasma(n=5)
82-99%
84-91%
83-94%
91-101%
heparin plasma(n=5)
84-99%
84-100%
81-100%
80-90%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only