Product name
Rat IL-18(Interleukin-18) ELISA Kit
Catalogue No.
ER0036
Detection method
Sandwich ELISA, Double Antibody
Application
IL-18 ELISA Kit allows for the in vitro quantitative determination of IL-18 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-18 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-18 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-97
93
EDTA plasma(n=5)
90-103
97
heparin plasma(n=5)
94-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
97-105%
88-100%
88-104%
86-93%
EDTA plasma(n=5)
83-93%
87-101%
84-95%
82-98%
heparin plasma(n=5)
80-99%
82-91%
80-93%
83-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only