Product name

Rat IL-18(Interleukin-18) ELISA Kit

Catalogue No.

ER0036

Detection method

Sandwich ELISA, Double Antibody

Application

IL-18 ELISA Kit allows for the in vitro quantitative determination of IL-18 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-18 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-18 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-97 93
EDTA plasma(n=5) 90-103 97
heparin plasma(n=5) 94-102 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 88-100% 88-104% 86-93%
EDTA plasma(n=5) 83-93% 87-101% 84-95% 82-98%
heparin plasma(n=5) 80-99% 82-91% 80-93% 83-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Rat IL-18(Interleukin-18) ELISA Kit

SKU: ER0036
$360Price
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