Product name | Rat IL-18(Interleukin-18) ELISA Kit |
Catalogue No. | ER0036 |
Detection method | Sandwich ELISA, Double Antibody |
Application | IL-18 ELISA Kit allows for the in vitro quantitative determination of IL-18 concentrations in plasma, tissue homogenates and other biological fluids. |
Size | 96T |
Range | 31.25-2000pg/ml |
Sensitivity | < 18.75pg/ml |
Storage | 4 ℃ for 6 months |
Recovery | Matrices listed below were spiked with certain level of IL-18 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-18 in samples. |
Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. |
CV(%) | Intra-Assay: CV<8% Inter-Assay: CV<10% |
Note | For Research Use Only |
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-97 | 93 |
EDTA plasma(n=5) | 90-103 | 97 |
heparin plasma(n=5) | 94-102 | 97 |
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 97-105% | 88-100% | 88-104% | 86-93% |
EDTA plasma(n=5) | 83-93% | 87-101% | 84-95% | 82-98% |
heparin plasma(n=5) | 80-99% | 82-91% | 80-93% | 83-100% |