Product name
Rat IL-13(Interleukin-13) ELISA Kit
Catalogue No.
ER0034
Detection method
Sandwich ELISA, Double Antibody
Application
IL-13 ELISA Kit allows for the in vitro quantitative determination of IL-13 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-13 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-13 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
94
EDTA plasma(n=5)
88-99
92
heparin plasma(n=5)
86-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-13 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-103%
94-104%
85-104%
85-100%
EDTA plasma(n=5)
83-99%
83-97%
82-93%
87-99%
heparin plasma(n=5)
86-100%
82-95%
82-95%
81-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only