Product name
Rat IL-12/p40 (Interleukin 12 p40) ELISA Kit
Catalogue No.
ER1087
Detection method
Sandwich ELISA, Double Antibody
Application
IL-12/p40 ELISA Kit allows for the in vitro quantitative determination of IL-12/p40 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
11.719-750pg/ml
Sensitivity
< 7.031pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-12/p40 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12/p40 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
93
EDTA plasma(n=5)
85-104
98
heparin plasma(n=5)
86-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12/p40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
94-102%
86-99%
97-104%
87-103%
EDTA plasma(n=5)
82-99%
82-94%
83-93%
83-99%
heparin plasma(n=5)
80-99%
82-90%
81-96%
90-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only