Product name

Rat IL-12/p40 (Interleukin 12 p40) ELISA Kit

Catalogue No.

ER1087

Detection method

Sandwich ELISA, Double Antibody

Application

IL-12/p40 ELISA Kit allows for the in vitro quantitative determination of IL-12/p40 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

11.719-750pg/ml

Sensitivity

< 7.031pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-12/p40 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12/p40 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-102 93
EDTA plasma(n=5) 85-104 98
heparin plasma(n=5) 86-104 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12/p40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 86-99% 97-104% 87-103%
EDTA plasma(n=5) 82-99% 82-94% 83-93% 83-99%
heparin plasma(n=5) 80-99% 82-90% 81-96% 90-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Rat IL-12/p40 (Interleukin 12 p40) ELISA Kit

SKU: ER1087
$360Price
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