Product name

Rat IL-12 (Interleukin 12) ELISA Kit

Catalogue No.

ER1086

Detection method

Sandwich ELISA, Double Antibody

Application

IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-103 92
EDTA plasma(n=5) 90-104 99
heparin plasma(n=5) 85-99 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 92-103% 90-104% 87-100%
EDTA plasma(n=5) 85-98% 82-95% 84-98% 85-100%
heparin plasma(n=5) 84-96% 82-98% 84-100% 85-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Rat IL-12 (Interleukin 12) ELISA Kit

SKU: ER1086
$360Price
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