Specifications
Product name
Rat Ifna1(Interferon alpha-1) ELISA Kit
Catalogue No.
ER0179
Detection method
Sandwich ELISA, Double Antibody
Application
Ifna1 ELISA Kit allows for the in vitro quantitative determination of Ifna1 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.8-500pg/ml
Sensitivity
< 4.688pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Ifna1 and the recovery rates were calculated by comparing the measured value to the expected amount of Ifna1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-100
92
EDTA plasma(n=5)
86-105
92
heparin plasma(n=5)
87-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ifna1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
87-105%
86-102%
86-104%
91-105%
EDTA plasma(n=5)
84-92%
83-99%
82-98%
84-101%
heparin plasma(n=5)
81-95%
81-95%
81-97%
82-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only