Specifications
Product name
Rat IFN-β (Interferon Beta) ELISA Kit
Catalogue No.
ER1071
Detection method
Sandwich ELISA, Double Antibody
Application
IFN-β ELISA Kit allows for the in vitro quantitative determination of IFN-β concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFN-β and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-β in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-98
93
EDTA plasma(n=5)
88-104
94
heparin plasma(n=5)
92-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
85-104%
92-105%
92-101%
85-102%
EDTA plasma(n=5)
86-98%
89-101%
84-100%
89-90%
heparin plasma(n=5)
87-99%
80-100%
88-98%
83-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only