Specifications
Product name | Rat IFN-β (Interferon Beta) ELISA Kit |
Catalogue No. | ER1071 |
Detection method | Sandwich ELISA, Double Antibody |
Application | IFN-β ELISA Kit allows for the in vitro quantitative determination of IFN-β concentrations in plasma, tissue homogenates and other biological fluids. |
Size | 96T |
Range | 15.625-1000pg/ml |
Sensitivity | < 9.375pg/ml |
Storage | 4 ℃ for 6 months |
Recovery | Matrices listed below were spiked with certain level of IFN-β and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-β in samples. |
Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. |
CV(%) | Intra-Assay: CV<8% Inter-Assay: CV<10% |
Note | For Research Use Only |
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 85-98 | 93 |
EDTA plasma(n=5) | 88-104 | 94 |
heparin plasma(n=5) | 92-105 | 99 |
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 85-104% | 92-105% | 92-101% | 85-102% |
EDTA plasma(n=5) | 86-98% | 89-101% | 84-100% | 89-90% |
heparin plasma(n=5) | 87-99% | 80-100% | 88-98% | 83-99% |