Specifications
Product name
Rat IFN-α (Interferon α) ELISA Kit
Catalogue No.
ER1070
Detection method
Sandwich ELISA, Double Antibody
Application
IFN-α ELISA Kit allows for the in vitro quantitative determination of IFN-α concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFN-α and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-α in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
91
EDTA plasma(n=5)
85-105
95
heparin plasma(n=5)
85-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
88-103%
85-103%
90-102%
90-105%
EDTA plasma(n=5)
82-94%
86-101%
82-98%
90-101%
heparin plasma(n=5)
85-100%
84-100%
89-99%
80-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only