Product name
Mouse IL-1b(Interleukin 1 Beta) ELISA Kit
Catalogue No.
EM0109
Alias
IL-1b ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IL-1b ELISA Kit allows for the in vitro quantitative determination of IL-1β(Interleukin 1 Beta)/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
12.5-800pg/ml
Sensitivity
< 7.5pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1β(Interleukin 1 Beta)/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β(Interleukin 1 Beta)/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-102
96
EDTA plasma(n=5)
88-104
94
heparin plasma(n=5)
89-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β(Interleukin 1 Beta)/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
90-104%
88-103%
86-104%
98-101%
EDTA plasma(n=5)
82-100%
89-101%
84-98%
86-101%
heparin plasma(n=5)
84-99%
80-94%
84-95%
82-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only