Product name | Mouse IL-1b(Interleukin 1 Beta) ELISA Kit |
Catalogue No. | EM0109 |
Alias | IL-1b ELISA Kit |
Detection method | Sandwich ELISA, Double Antibody |
Application | IL-1b ELISA Kit allows for the in vitro quantitative determination of IL-1β(Interleukin 1 Beta)/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin concentrations in plasma, tissue homogenates and other biological fluids. |
Size | 96T |
Range | 12.5-800pg/ml |
Sensitivity | < 7.5pg/ml |
Storage | 4 ℃ for 6 months |
Recovery | Matrices listed below were spiked with certain level of IL-1β(Interleukin 1 Beta)/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β(Interleukin 1 Beta)/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin in samples. |
Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β(Interleukin 1 Beta)/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. |
CV(%) | Intra-Assay: CV<8% Inter-Assay: CV<10% |
Note | For Research Use Only |
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 91-102 | 96 |
EDTA plasma(n=5) | 88-104 | 94 |
heparin plasma(n=5) | 89-103 | 97 |
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 90-104% | 88-103% | 86-104% | 98-101% |
EDTA plasma(n=5) | 82-100% | 89-101% | 84-98% | 86-101% |
heparin plasma(n=5) | 84-99% | 80-94% | 84-95% | 82-95% |