Product name

Mouse IL-1a(Interleukin 1 Alpha) ELISA Kit

Catalogue No.

EM0108

Alias

IL-1a ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-1a ELISA Kit allows for the in vitro quantitative determination of IL-1α/IL-1alpha/IL1A/IL1-A/IL1/IL1F1/Preinterleukin 1 Alpha/Hematopoietin-1/Pro-Interleukin-1-Alpha concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-1α/IL-1alpha/IL1A/IL1-A/IL1/IL1F1/Preinterleukin 1 Alpha/Hematopoietin-1/Pro-Interleukin-1-Alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1α/IL-1alpha/IL1A/IL1-A/IL1/IL1F1/Preinterleukin 1 Alpha/Hematopoietin-1/Pro-Interleukin-1-Alpha in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-99 91
EDTA plasma(n=5) 88-102 93
heparin plasma(n=5) 87-101 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1α/IL-1alpha/IL1A/IL1-A/IL1/IL1F1/Preinterleukin 1 Alpha/Hematopoietin-1/Pro-Interleukin-1-Alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-99% 87-104% 88-103% 86-105%
EDTA plasma(n=5) 82-101% 86-98% 82-98% 82-93%
heparin plasma(n=5) 83-97% 81-95% 80-100% 82-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Mouse IL-1a(Interleukin 1 Alpha) ELISA Kit

SKU: EM0108
$350Price
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