Product name | Mouse IL-1a(Interleukin 1 Alpha) ELISA Kit |
Catalogue No. | EM0108 |
Alias | IL-1a ELISA Kit |
Detection method | Sandwich ELISA, Double Antibody |
Application | IL-1a ELISA Kit allows for the in vitro quantitative determination of IL-1α/IL-1alpha/IL1A/IL1-A/IL1/IL1F1/Preinterleukin 1 Alpha/Hematopoietin-1/Pro-Interleukin-1-Alpha concentrations in plasma, tissue homogenates and other biological fluids. |
Size | 96T |
Range | 15.625-1000pg/ml |
Sensitivity | < 9.375pg/ml |
Storage | 4 ℃ for 6 months |
Recovery | Matrices listed below were spiked with certain level of IL-1α/IL-1alpha/IL1A/IL1-A/IL1/IL1F1/Preinterleukin 1 Alpha/Hematopoietin-1/Pro-Interleukin-1-Alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1α/IL-1alpha/IL1A/IL1-A/IL1/IL1F1/Preinterleukin 1 Alpha/Hematopoietin-1/Pro-Interleukin-1-Alpha in samples. |
Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1α/IL-1alpha/IL1A/IL1-A/IL1/IL1F1/Preinterleukin 1 Alpha/Hematopoietin-1/Pro-Interleukin-1-Alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. |
CV(%) | Intra-Assay: CV<8% Inter-Assay: CV<10% |
Note | For Research Use Only |
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 86-99 | 91 |
EDTA plasma(n=5) | 88-102 | 93 |
heparin plasma(n=5) | 87-101 | 97 |
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 86-99% | 87-104% | 88-103% | 86-105% |
EDTA plasma(n=5) | 82-101% | 86-98% | 82-98% | 82-93% |
heparin plasma(n=5) | 83-97% | 81-95% | 80-100% | 82-97% |