Product name
Mouse IL-18 (Interleukin 18) ELISA Kit
Catalogue No.
EM1158
Alias
IL-18 ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IL-18 ELISA Kit allows for the in vitro quantitative determination of IL-18(Interleukin 18)/IL18/IGIF/IL-1g/IL-1F4/Interferon-gamma-inducing factor concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-18(Interleukin 18)/IL18/IGIF/IL-1g/IL-1F4/Interferon-gamma-inducing factor and the recovery rates were calculated by comparing the measured value to the expected amount of IL-18(Interleukin 18)/IL18/IGIF/IL-1g/IL-1F4/Interferon-gamma-inducing factor in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
95
EDTA plasma(n=5)
85-103
94
heparin plasma(n=5)
85-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18(Interleukin 18)/IL18/IGIF/IL-1g/IL-1F4/Interferon-gamma-inducing factor and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
88-103%
85-105%
86-90%
85-105%
EDTA plasma(n=5)
85-100%
82-100%
82-101%
82-101%
heparin plasma(n=5)
83-94%
90-98%
82-97%
80-88%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only