Specifications
Product name
Mouse IFNγ(Interferon Gamma) ELISA Kit
Catalogue No.
EM0093
Alias
IFNγ ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IFNγ ELISA Kit allows for the in vitro quantitative determination of IFN-γ(Interferon Gamma)/IFNG/IFG/IFI/Type II Interferon concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFN-γ(Interferon Gamma)/IFNG/IFG/IFI/Type II Interferon and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-γ(Interferon Gamma)/IFNG/IFG/IFI/Type II Interferon in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
93
EDTA plasma(n=5)
85-96
91
heparin plasma(n=5)
85-100
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-γ(Interferon Gamma)/IFNG/IFG/IFI/Type II Interferon and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
95-99%
92-104%
85-103%
91-104%
EDTA plasma(n=5)
82-100%
84-101%
83-100%
83-100%
heparin plasma(n=5)
80-100%
84-95%
89-98%
84-94%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only