Product description
Contents
Solution DS: 30 ml
Solution MS: 40 ml
Proteinase K: 2 ml
Wash Buffer PS: 60 ml
Wash Buffer PE: 30 ml
Eluent Buffer TE: 10 ml
Medico™ Spin column: 100 each
Description:
The Medico™ Quick Tissue/Culture Cells Genomic DNA Extraction kit provides a simple and rapid method for high quality genomic DNA purification from mammalian tissues (either fresh or frozen at -70°C until use) and culture cells. The Medico™ Quick Tissue/Culture Cells Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-35 µg from 10 mg of tissue or 1 x 106-107cultrue cells. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.
Features: Efficient: 3-35 µg of genomic DNA from 10 mg of tissue or 1 x 106-107 cultrue cells.
Fast:
Procedure takes only 30 min. Universal: purifies genomic DNA from various sources. Safe: No phenol/cholroform extraction step. High purity: Purified DNA is ready for downstream application such as PCR, restriction digestion. Downstream Applications: Purified DNA is free from contaminants and enzyme inhibitors, and typically has A260/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:
Restriction digestion
PCR
Labeling
Library construction
Storage: Store Protein K at -20℃, other reagents can be stored at room temperature for up to 1 year. Any precipitate in the Solution DS and Solution MS can be re-dissolved by incubating at 37°C before use.
Important Notes:
• Examine the solution for precipitates before each use. Re-dissolve any precipitate by warming the solution to 37°C and cooling to 25°C.• All purification steps should be carried out at room temperature. • Prior to the initial use of the kit, dilute the Wash Buffer(PE) with thanol (96-100%):
After the ethanol has been added, mark the check box on the bottle to indicate the completed step.
| 100 preps |
|
Wash Buffer(PE) | 30 ml |
|
Ethanol | 90 ml |
|
Total Volume | 120 ml |
|