- Product description
Contents
- Solution DS: 30 ml
- Solution MS: 40 ml
- Proteinase K: 2 ml
- Wash Buffer PS: 60 ml
- Wash Buffer PE: 30 ml
- Eluent Buffer TE: 10 ml
- Medico™ Spin column: 100 each
Description:
The Medico™ Quick Tissue/Culture Cells Genomic DNA Extraction kit provides a simple and rapid method for high quality genomic DNA purification from mammalian tissues (either fresh or frozen at -70°C until use) and culture cells. The Medico™ Quick Tissue/Culture Cells Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-35 µg from 10 mg of tissue or 1 x 106-107cultrue cells. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.
Features:
Efficient: 3-35 µg of genomic DNA from 10 mg of tissue or 1 x 106-107 cultrue cells.
Fast:
Procedure takes only 30 min.
Universal: purifies genomic DNA from various sources.
Safe: No phenol/cholroform extraction step.
High purity: Purified DNA is ready for downstream application such as PCR, restriction digestion.
Downstream Applications:
Purified DNA is free from contaminants and enzyme inhibitors, and typically has A260/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:
- Restriction digestion
- PCR
- Labeling
- Library construction
Storage:
Store Protein K at -20℃, other reagents can be stored at room temperature for up to 1 year. Any precipitate in the Solution DS and Solution MS can be re-dissolved by incubating at 37°C before use.
Important Notes:
• Examine the solution for precipitates before each use. Re-dissolve any precipitate by warming the solution to 37°C and cooling to 25°C.• All purification steps should be carried out at room temperature.
• Prior to the initial use of the kit, dilute the Wash Buffer(PE) with thanol (96-100%):
After the ethanol has been added, mark the check box on the bottle to indicate the completed step.
100 preps |
| |
Wash Buffer(PE) | 30 ml |
|
Ethanol | 90 ml |
|
Total Volume | 120 ml |
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