Product description
Contents:
Size 25 preps Size: 50 preps
Amount (size) 100 ul (5,000U) 200 ul (10,000U)
M-MLV): 100 ul 200 ul
5x First-strand Buffer: 100 ul 100 ul
Description The Medico M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Dongsheng M-MLV Reverse Transcriptase has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase. Source E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase. Applications Generation of first strand cDNA for use in:
PCR, see Protocol for First-strand cDNA Synthesis;
real-time PCR;
second strand cDNA synthesis;
DNA labeling;
Analysis of RNA by primer extension.
Quality Control The absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.
Functionally tested in first strand cDNA synthesis. Store Buffer 20m M Tris-HCl (pH 7.5) 200 mM NaCl 0.1 mM EDTA 1 mM DTT 0.01% NP-40 50% glycerol 5xFirst-Strand Buffer 250 mM Tris-HCl (pH 8.3 at 25℃) 375 mM KCl 15 mM MgCl2 50 mM DTT