Product name
Human IL-1beta(Interleukin 1 Beta) ELISA Kit
Catalogue No.
EH0185
Alias
IL-1β ELISA Kit, IL-1beta ELISA Kit, IL1B ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, catabolin ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IL-1beta ELISA Kit allows for the in vitro quantitative determination of IL-1beta concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.9-250pg/ml
Sensitivity
< 2.344pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1beta and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1beta in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
94
EDTA plasma(n=5)
85-102
94
heparin plasma(n=5)
92-96
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1beta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
85-104%
90-101%
92-103%
87-100%
EDTA plasma(n=5)
82-99%
88-98%
87-99%
84-101%
heparin plasma(n=5)
80-98%
80-97%
80-100%
80-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only