Product name
Human IL-1alpha(Interleukin 1 Alpha) ELISA Kit
Catalogue No.
EH0184
Alias
IL-1α ELISA Kit, IL-1alpha ELISA Kit, IL1A ELISA Kit, IL1-A ELISA Kit, IL1 ELISA Kit, IL1F1 ELISA Kit, Preinterleukin 1 Alpha ELISA Kit, Hematopoietin-1 ELISA Kit, Pro-Interleukin-1-Alpha ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IL-1alpha ELISA Kit allows for the in vitro quantitative determination of IL-1alpha concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.906-250pg/ml
Sensitivity
< 2.344pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1alpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
95
EDTA plasma(n=5)
88-98
94
heparin plasma(n=5)
89-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
90-105%
91-105%
85-98%
85-101%
EDTA plasma(n=5)
86-101%
82-98%
86-100%
82-91%
heparin plasma(n=5)
88-94%
86-98%
83-98%
88-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only