Product name
Human IL-17E/IL-25(Interleukin 25) ELISA Kit
Catalogue No.
EH0180
Alias
IL-25 ELISA Kit, IL-17E ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IL-17E/IL-25 ELISA Kit allows for the in vitro quantitative determination of IL-17E/IL-25 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.6-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-17E/IL-25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-17E/IL-25 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
95
EDTA plasma(n=5)
85-102
94
heparin plasma(n=5)
85-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-17E/IL-25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
85-103%
88-92%
85-101%
85-101%
EDTA plasma(n=5)
82-99%
82-100%
82-94%
82-94%
heparin plasma(n=5)
81-97%
87-95%
85-95%
80-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only