Product name
Human IL-12(p40)(Interleukin 12 p40) ELISA Kit
Catalogue No.
EH0175
Alias
IL-12(p40) ELISA Kit, IL12B ELISA Kit, IL-12B ELISA Kit, CLMF2 ELISA Kit, NKSF2 ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12(p40) concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-12(p40) and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12(p40) in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
93
EDTA plasma(n=5)
85-105
99
heparin plasma(n=5)
86-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12(p40) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
88-103%
85-103%
90-95%
85-105%
EDTA plasma(n=5)
84-100%
84-90%
87-99%
83-101%
heparin plasma(n=5)
80-98%
83-95%
87-99%
80-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only