Product name

Human IL-12 (Interleukin 12) ELISA Kit

Catalogue No.

EH0176

Alias

Interleukin 12 p70 ELISA Kit, CLMF p35 ELISA Kit, Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit, IL-12 subunit p35 ELISA Kit, IL-12, subunit p35 ELISA Kit, interleukin 12, p35 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-105 97
EDTA plasma(n=5) 86-103 94
heparin plasma(n=5) 85-95 90
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-98% 85-103% 87-101% 93-105%
EDTA plasma(n=5) 83-92% 82-96% 91-97% 83-101%
heparin plasma(n=5) 83-97% 80-100% 83-95% 81-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Human IL-12(Interleukin 12) ELISA Kit

SKU: EH0176
$350Price
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