Specifications
Product name
Human IFN-α (Interferon Alpha) ELISA Kit
Catalogue No.
EH3252
Alias
IFN-α(Interferon Alpha) ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IFN-α ELISA Kit allows for the in vitro quantitative determination of IFN-α concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFN-α and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-α in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
94
EDTA plasma(n=5)
89-104
98
heparin plasma(n=5)
86-94
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
87-104%
85-105%
88-99%
86-105%
EDTA plasma(n=5)
87-100%
84-99%
82-100%
88-101%
heparin plasma(n=5)
83-94%
82-100%
80-96%
84-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only