Name: | GAPDH Mouse Monoclonal |
Description: | Mouse Monoclonal Antibody to GAPDH |
Specificity: | Human, Mouse , Rat, Zebrafish |
Applications: | ELISA, IHC, WB, IF, FC, IP |
Immunogen: | glyceraldehyde-3-phosphate dehydrogenase (Calculated MW:36 kDa) |
Host: | Mouse |
Clonality: | Monoclonal |
Isotype: | IgG1 |
Conjugate: | Unconjugated |
Purity: | Protein A+G purification (≥95% as determined by SDS-PAGE) |
Product Form: | Liquid |
Formulation: | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.) |
Recommended dilution | WB: 1:5000-1:20000; IP: 1:3000-1:20000; IHC: 1:50-1:500; IF: 1:20-1:200 |
Background |
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3′-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. |
Uniprot information:
Gene Name | GAPDH |
Uniprot ID | P04406 |
Target:
Synonyms: | 38 kDa BFA-dependent ADP-ribosylation substrate Antibody aging associated gene 9 protein Antibody Aging-associated gene 9 protein Antibody BARS-38 Antibody cb609 Antibody EC 1.2.1.12 Antibody Epididymis secretory sperm binding protein Li 162eP Antibody G3PD Antibody G3PDH Antibody G3P_HUMAN Antibody GAPD Antibody Glyceraldehyde 3 phosphate dehydrogenase Antibody Glyceraldehyde-3-phosphate dehydrogenase Antibody HEL S 162eP Antibody HEL-S-162eP Antibody KNC-NDS6 Antibody MGC102544 Antibody MGC102546 Antibody MGC103190 Antibody MGC103191 Antibody MGC105239 Antibody MGC127711 Antibody MGC88685 Antibody OCAS, p38 component Antibody OCT1 coactivator in S phase, 38-KD component Antibody peptidyl cysteine S nitrosylase GAPDH Antibody Peptidyl-cysteine S-nitrosylase GAPDH Antibody wu:fb33a10 Antibody |
Research Areas:
Neuroscience → Neurology process → Neurodegenerative disease → Huntington's disease
Neuroscience → Neurology process → Neurodegenerative disease → Alzheimer's disease → OtherIsotype/Loading Controls → Loading Controls → GAPDHSignal Transduction → Metabolism → Energy MetabolismCancer → Cancer Metabolism → Metabolic signaling pathway → Metabolism of carbohydratesMetabolism → Pathways and Processes → Metabolic signaling pathways → Carbohydrate metabolismMetabolism → Pathways and Processes → Metabolic signaling pathways → Energy transfer pathways → Energy MetabolismMetabolism → Types of disease → Neurodegenerative disease
Please Note: GAPDH Mouse Monoclonal is for research use only. It is not intended for diagnostic or therapeutic use.