Product description

Contents
Fast Taq DNA Polymerase (5 U/μl): 50 μl
10xFast PCR Buffer (Mg2+ Plus): 1.25 ml
dNTPs (each 2.5 mM): 1 ml
6xLoading Buffer: 1 ml
 
Note 
• Fast Taq DNA Polymerase has two concentrations with 2.5 U/μl and 5 U/μl. Default package is 5
U/μl.
• 10×FastPCR Buffer (Mg2+ Plus) can replace with 10×PCR Buffer (Mg2+ free) and 25 mM MgCl2.
 
Description
Fast Taq DNA polymerase, developed by Medico. is the latest generation Taq-based
DNA polymerase. It possesses high amplification efficiency as Taq polymerase does, and fast elongation ability as KOD polymerase does, can be used in a variety of PCR. The Fast PCR Buffer , designed for Fast Taq DNA polymerase, can be used in fast amplification reaction.The elongation rate of Fast Taq DNA polymerase is 2-fold higher than the one of regular Taq DNA polymerase, which shortens the amplification time by half .

Features
Fast rate of elongation: elongation velocity can reach to 3 kb/min, 2x higher than regular Taq DNA polymerase
Highly thermostable : have a half-life of over 40 min at  95°C incubation Generates 3'-dA overhangs PCR products

Applications:
Routine PCR
PCR labeling
PCR sequencing
Generate PCR product for TA cloning

Unit Definition:
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Quality Control:
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests Functionally tested in amplification of a single-copy gene from human genomic DNA

Storage Buffer
20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 %TW 20, 0.5 % NP 40, 50 % Glycerol

10X Fast PCR Buffer
200 mM Tris-Cl(PH 8.8), 100 mM KCl, 100mM (NH4)2SO4, 16 mM MgSO4, 1% Triton-X-100.
 
Store all components at –20°C