- Fast Taq DNA Polymerase (5 U/μl): 50 μl
- 10xFast PCR Buffer (Mg2+ Plus): 1.25 ml
- dNTPs (each 2.5 mM): 1 ml
- 6xLoading Buffer: 1 ml
• Fast Taq DNA Polymerase has two concentrations with 2.5 U/μl and 5 U/μl. Default package is 5
• 10×FastPCR Buffer (Mg2+ Plus) can replace with 10×PCR Buffer (Mg2+ free) and 25 mM MgCl2.
Fast Taq DNA polymerase, developed by Medico. is the latest generation Taq-based
DNA polymerase. It possesses high amplification efficiency as Taq polymerase does, and fast elongation ability as KOD polymerase does, can be used in a variety of PCR. The Fast PCR Buffer , designed for Fast Taq DNA polymerase, can be used in fast amplification reaction.The elongation rate of Fast Taq DNA polymerase is 2-fold higher than the one of regular Taq DNA polymerase, which shortens the amplification time by half .
Fast rate of elongation: elongation velocity can reach to 3 kb/min, 2x higher than regular Taq DNA polymerase
Highly thermostable : have a half-life of over 40 min at 95°C incubation Generates 3'-dA overhangs PCR products
Generate PCR product for TA cloning
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests Functionally tested in amplification of a single-copy gene from human genomic DNA
20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 %TW 20, 0.5 % NP 40, 50 % Glycerol
10X Fast PCR Buffer
200 mM Tris-Cl(PH 8.8), 100 mM KCl, 100mM (NH4)2SO4, 16 mM MgSO4, 1% Triton-X-100.
Store all components at –20°C
Fast Taq DNA Polymerase
Ships in: 5 Business Days