Product name

Rat IL-1β (Interleukin 1 Beta) ELISA Kit

Catalogue No.

ER1094

Detection method

Sandwich ELISA, Double Antibody

Application

IL-1β ELISA Kit allows for the in vitro quantitative determination of IL-1β concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-1β and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-105 95
EDTA plasma(n=5) 87-99 93
heparin plasma(n=5) 86-104 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 86-96% 85-105% 88-105%
EDTA plasma(n=5) 88-98% 83-99% 85-100% 86-101%
heparin plasma(n=5) 83-92% 84-99% 84-95% 87-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Rat IL-1β (Interleukin 1 Beta) ELISA Kit

SKU: ER1094
$360Price
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