• Specifications
Product name

Rat IFN-α (Interferon α) ELISA Kit

Catalogue No.

ER1070

Detection method

Sandwich ELISA, Double Antibody

Application

IFN-α ELISA Kit allows for the in vitro quantitative determination of IFN-α concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IFN-α and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-α in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 91
EDTA plasma(n=5) 85-105 95
heparin plasma(n=5) 85-102 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 85-103% 90-102% 90-105%
EDTA plasma(n=5) 82-94% 86-101% 82-98% 90-101%
heparin plasma(n=5) 85-100% 84-100% 89-99% 80-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Rat IFN-α (Interferon α) ELISA Kit

SKU: ER1070
$360Price
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