Specifications
Product name
Rabbit IFN-γ (Interferon Gamma) ELISA Kit
Catalogue No.
ERB0051
Detection method
Sandwich ELISA, Double Antibody
Application
IFN-γ ELISA Kit allows for the in vitro quantitative determination of IFN-γ concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFN-γ and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-γ in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
95
EDTA plasma(n=5)
87-105
97
heparin plasma(n=5)
86-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
95-103%
92-100%
89-99%
91-104%
EDTA plasma(n=5)
95-100%
86-101%
85-92%
86-100%
heparin plasma(n=5)
86-98%
84-94%
81-98%
80-93%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only