Specifications
	
		
			
Product name
			
			
Mouse G-CSF(Granulocyte Colony Stimulating Factor) ELISA Kit
			
		
		
			
Catalogue No.
			
			
EM0086
			
		
		
			
Alias
			
			
G-CSF ELISA Kit
			
		
		
			
Detection method
			
			
Sandwich ELISA, Double Antibody
			
		
		
			
Application
			
			
G-CSF ELISA Kit allows for the in vitro quantitative determination of G-CSF/CSF3/C17orf33/CSF3OS/Pluripoietin/Filgrastim/Lenograstim concentrations in plasma, tissue homogenates and other biological fluids.
			
		
		
			
Size
			
			
96T
			
		
		
			
Range
			
			
15.625-1000pg/ml
			
		
		
			
Sensitivity
			
			
< 9.375pg/ml
			
		
		
			
Storage
			
			
4 ℃ for 6 months
			
		
		
			
Recovery
			
			
Matrices listed below were spiked with certain level of G-CSF/CSF3/C17orf33/CSF3OS/Pluripoietin/Filgrastim/Lenograstim and the recovery rates were calculated by comparing the measured value to the expected amount of G-CSF/CSF3/C17orf33/CSF3OS/Pluripoietin/Filgrastim/Lenograstim in samples.
			
				
					
						
Matrix
						
Recovery range(%)
						
Average(%)
					
					
						
serum(n=5)
						
87-103
						
95
					
					
						
EDTA plasma(n=5)
						
91-102
						
96
					
					
						
heparin plasma(n=5)
						
94-105
						
100
					
				
			
			
		
		
			
Linearity
			
			
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of G-CSF/CSF3/C17orf33/CSF3OS/Pluripoietin/Filgrastim/Lenograstim and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
			
				
					
						
Sample
						
1:2
						
1:4
						
1:8
						
1:16
					
					
						
serum(n=5)
						
87-101%
						
85-103%
						
85-105%
						
85-102%
					
					
						
EDTA plasma(n=5)
						
83-92%
						
82-99%
						
82-98%
						
82-101%
					
					
						
heparin plasma(n=5)
						
80-96%
						
82-99%
						
86-99%
						
89-98%
					
				
			
			
		
		
			
CV(%)
			
			
Intra-Assay: CV<8%
			Inter-Assay: CV<10%
			
		
		
			
Note
			
			
For Research Use Only