Product name

Human MIP-3β(Macrophage Inflammatory Protein 3β) ELISA Kit

Catalogue No.

EH0002

Alias

MIP-3β ELISA Kit, ELC ELISA Kit, CCL19 ELISA Kit, beta chemokine exodus-3 ELISA Kit, Beta-chemokine exodus-3 ELISA Kit, CC chemokine ligand 19 ELISA Kit, C-C motif chemokine 19 ELISA Kit, chemokine(C-C motif) ligand 19 ELISA Kit, CKb11 ELISA Kit, EBI1-ligand chemokine ELISA Kit, ELCMIP-3-beta ELISA Kit, Epstein-Barr virus-induced molecule 1 ligand chemokine ELISA Kit, exodus-3 ELISA Kit, Macrophage inflammatory protein 3 beta ELISA Kit, macrophage inflammatory protein 3-beta ELISA Kit, MGC34433 ELISA Kit, MIP-3b ELISA Kit, MIP3BCK beta-11 ELISA Kit, SCYA19EBI1 ligand chemokine ELISA Kit, small inducible cytokine subfamily A(Cys-Cys), member 19 ELISA Kit, Small-inducible cytokine A19 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MIP-3β ELISA Kit allows for the in vitro quantitative determination of MIP-3β concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MIP-3β and the recovery rates were calculated by comparing the measured value to the expected amount of MIP-3β in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-102 92
EDTA plasma(n=5) 85-100 93
heparin plasma(n=5) 92-105 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP-3β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-102% 85-103% 87-102% 87-103%
EDTA plasma(n=5) 83-98% 91-100% 85-101% 87-99%
heparin plasma(n=5) 85-96% 83-100% 80-99% 83-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Human MIP-3β(Macrophage Inflammatory Protein 3β) ELISA Kit

SKU: EH0002
$440Price
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    Principle of the Assay:

    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MIP-3
    βantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MIP-3β
    antibody was used as detection antibodies. The standards, test samples and biotin conjugated
    detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin
    was added and unbound conjugates were washed away with wash buffer. TMB
    substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to
    produce a blue color product that changed into yellow after adding acidic stop solution. The
    density of yellow is proportional to the MIP-3β amount of sample captured in plate. Read the
    O.D. absorbance at 450nm in a microplate reader, and then the concentration of MIP-3β can
    be calculated.

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