Human MIP-3β(Macrophage Inflammatory Protein 3β) ELISA Kit
			
			Product name						
Human MIP-3β(Macrophage Inflammatory Protein 3β) ELISA Kit
							
		
			Catalogue No.						
EH0002
							
		
			Alias						
MIP-3β ELISA Kit, ELC ELISA Kit, CCL19 ELISA Kit, beta chemokine exodus-3 ELISA Kit, Beta-chemokine exodus-3 ELISA Kit, CC chemokine ligand 19 ELISA Kit, C-C motif chemokine 19 ELISA Kit, chemokine(C-C motif) ligand 19 ELISA Kit, CKb11 ELISA Kit, EBI1-ligand chemokine ELISA Kit, ELCMIP-3-beta ELISA Kit, Epstein-Barr virus-induced molecule 1 ligand chemokine ELISA Kit, exodus-3 ELISA Kit, Macrophage inflammatory protein 3 beta ELISA Kit, macrophage inflammatory protein 3-beta ELISA Kit, MGC34433 ELISA Kit, MIP-3b ELISA Kit, MIP3BCK beta-11 ELISA Kit, SCYA19EBI1 ligand chemokine ELISA Kit, small inducible cytokine subfamily A(Cys-Cys), member 19 ELISA Kit, Small-inducible cytokine A19 ELISA Kit
							
		
			Detection method						
Sandwich ELISA, Double Antibody
							
		
			Application						
MIP-3β ELISA Kit allows for the in vitro quantitative determination of MIP-3β concentrations in plasma, tissue homogenates and other biological fluids.
							
		
			Size						
96T
							
		
			Range						
15.625-1000pg/ml
							
		
			Sensitivity						
< 9.375pg/ml
							
		
			Storage						
4 ℃ for 6 months
							
		
			Recovery						
Matrices listed below were spiked with certain level of MIP-3β and the recovery rates were calculated by comparing the measured value to the expected amount of MIP-3β in samples.
			
									
						Matrix						Recovery range(%)						Average(%)					
					
						serum(n=5)						85-102						92					
					
						EDTA plasma(n=5)						85-100						93					
					
						heparin plasma(n=5)						92-105						99					
							
							
		
			Linearity						
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP-3β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
			
									
						Sample						1:2						1:4						1:8						1:16					
					
						serum(n=5)						89-102%						85-103%						87-102%						87-103%					
					
						EDTA plasma(n=5)						83-98%						91-100%						85-101%						87-99%					
					
						heparin plasma(n=5)						85-96%						83-100%						80-99%						83-100%					
							
							
		
			CV(%)						
Intra-Assay: CV<8%
			Inter-Assay: CV<10%
							
		
			Note						
For Research Use Only

Human MIP-3β(Macrophage Inflammatory Protein 3β) ELISA Kit

SKU: EH0002
$440Price

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    Principle of the Assay:

    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MIP-3
    βantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MIP-3β
    antibody was used as detection antibodies. The standards, test samples and biotin conjugated
    detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin
    was added and unbound conjugates were washed away with wash buffer. TMB
    substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to
    produce a blue color product that changed into yellow after adding acidic stop solution. The
    density of yellow is proportional to the MIP-3β amount of sample captured in plate. Read the
    O.D. absorbance at 450nm in a microplate reader, and then the concentration of MIP-3β can
    be calculated.