• Specifications
Product name

Human IFN-α (Interferon Alpha) ELISA Kit

Catalogue No.

EH3252

Alias

IFN-α(Interferon Alpha) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IFN-α ELISA Kit allows for the in vitro quantitative determination of IFN-α concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IFN-α and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-α in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 94
EDTA plasma(n=5) 89-104 98
heparin plasma(n=5) 86-94 90
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-104% 85-105% 88-99% 86-105%
EDTA plasma(n=5) 87-100% 84-99% 82-100% 88-101%
heparin plasma(n=5) 83-94% 82-100% 80-96% 84-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Human IFN-α (Interferon Alpha) ELISA Kit

SKU: EH3252
$360Price
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