Specifications
	
		
			
Product name
			
			
Human GM-CSF Ab (Anti-Granulocyte Macrophage Colony Stimulating Factor Antibody) ELISA Kit
			
		
		
			
Catalogue No.
			
			
EH3153
			
		
		
			
Alias
			
			
GM-CSF Ab ELISA Kit
			
		
		
			
Detection method
			
			
Sandwich ELISA, Double Antigen
			
		
		
			
Application
			
			
GM-CSF Ab ELISA Kit allows for the in vitro quantitative determination of GM-CSF Ab concentrations in plasma, tissue homogenates and other biological fluids.
			
		
		
			
Size
			
			
96T
			
		
		
			
Range
			
			
0.625-40ng/ml
			
		
		
			
Sensitivity
			
			
< 0.375ng/ml
			
		
		
			
Storage
			
			
4 ℃ for 6 months
			
		
		
			
Recovery
			
			
Matrices listed below were spiked with certain level of GM-CSF Ab and the recovery rates were calculated by comparing the measured value to the expected amount of GM-CSF Ab in samples.
			
				
					
						
Matrix
						
Recovery range(%)
						
Average(%)
					
					
						
serum(n=5)
						
90-101
						
96
					
					
						
EDTA plasma(n=5)
						
85-99
						
93
					
					
						
heparin plasma(n=5)
						
86-104
						
99
					
				
			
			
		
		
			
Linearity
			
			
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GM-CSF Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
			
				
					
						
Sample
						
1:2
						
1:4
						
1:8
						
1:16
					
					
						
serum(n=5)
						
85-102%
						
91-104%
						
91-105%
						
89-101%
					
					
						
EDTA plasma(n=5)
						
85-96%
						
89-98%
						
90-100%
						
88-97%
					
					
						
heparin plasma(n=5)
						
80-98%
						
88-99%
						
82-100%
						
80-95%
					
				
			
			
		
		
			
CV(%)
			
			
Intra-Assay: CV<8%
			Inter-Assay: CV<10%
			
		
		
			
Note
			
			
For Research Use Only