Product name
			
			
Human ALCAM(CD166 antigen) ELISA Kit
			
		
		
			
Catalogue No.
			
			
EH0001
			
		
		
			
Alias
			
			
ALCAM ELISA Kit, CD166 ELISA Kit, MEMD ELISA Kit, activated leucocyte cell adhesion molecule ELISA Kit, CD166 antigen ELISA Kit
			
		
		
			
Detection method
			
			
Sandwich ELISA, Double Antibody
			
		
		
			
Application
			
			
ALCAM ELISA Kit allows for the in vitro quantitative determination of ALCAM concentrations in plasma, tissue homogenates and other biological fluids.
			
		
		
			
Size
			
			
96T
			
		
		
			
Range
			
			
62.5-4000pg/ml
			
		
		
			
Sensitivity
			
			
< 37.5pg/ml
			
		
		
			
Storage
			
			
4 ℃ for 6 months
			
		
		
			
Recovery
			
			
Matrices listed below were spiked with certain level of ALCAM and the recovery rates were calculated by comparing the measured value to the expected amount of ALCAM in samples.
			
				
					
						
Matrix
						
Recovery range(%)
						
Average(%)
					
					
						
serum(n=5)
						
85-105
						
92
					
					
						
EDTA plasma(n=5)
						
92-99
						
96
					
					
						
heparin plasma(n=5)
						
89-105
						
99
					
				
			
			
		
		
			
Linearity
			
			
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALCAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
			
				
					
						
Sample
						
1:2
						
1:4
						
1:8
						
1:16
					
					
						
serum(n=5)
						
87-105%
						
86-105%
						
86-103%
						
89-105%
					
					
						
EDTA plasma(n=5)
						
82-98%
						
85-101%
						
87-98%
						
82-97%
					
					
						
heparin plasma(n=5)
						
84-98%
						
80-94%
						
80-99%
						
82-96%
					
				
			
			
		
		
			
CV(%)
			
			
Intra-Assay: CV<8%
			Inter-Assay: CV<10%
			
		
		
			
Note
			
			
For Research Use Only