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MEDICO 

A Company In Developing Life Sciences Products for Research

COPYRIGHT© 2017 by Medico. All rights reserved.

Product name

Human ALCAM(CD166 antigen) ELISA Kit

Catalogue No.

EH0001

Alias

ALCAM ELISA Kit, CD166 ELISA Kit, MEMD ELISA Kit, activated leucocyte cell adhesion molecule ELISA Kit, CD166 antigen ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ALCAM ELISA Kit allows for the in vitro quantitative determination of ALCAM concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ALCAM and the recovery rates were calculated by comparing the measured value to the expected amount of ALCAM in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-105 92
EDTA plasma(n=5) 92-99 96
heparin plasma(n=5) 89-105 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALCAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 86-105% 86-103% 89-105%
EDTA plasma(n=5) 82-98% 85-101% 87-98% 82-97%
heparin plasma(n=5) 84-98% 80-94% 80-99% 82-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Human ALCAM(CD166 antigen) ELISA Kit

SKU: EB0001
$440Price
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    Principle of the Assay:

    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-
    ALCAMantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ALCAM
    antibody was used as detection antibodies. The standards, test samples and biotin conjugated
    detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin
    was added and unbound conjugates were washed away with wash buffer. TMB
    substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to
    produce a blue color product that changed into yellow after adding acidic stop solution. The
    density of yellow is proportional to the ALCAM amount of sample captured in plate. Read the
    O.D. absorbance at 450nm in a microplate reader, and then the concentration of ALCAM can be
    calculated.

     

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