						Product name 						Human ALCAM(CD166 antigen) ELISA Kit
			 		
					Catalogue No. 						EH0001
			 		
					Alias 						ALCAM ELISA Kit, CD166 ELISA Kit, MEMD ELISA Kit, activated leucocyte cell adhesion molecule ELISA Kit, CD166 antigen ELISA Kit
			 		
					Detection method 						Sandwich ELISA, Double Antibody
			 		
					Application 						ALCAM ELISA Kit allows for the in vitro quantitative determination of ALCAM concentrations in plasma, tissue homogenates and other biological fluids.
			 		
					Size 						96T
			 		
					Range 						62.5-4000pg/ml
			 		
					Sensitivity 						< 37.5pg/ml
			 		
					Storage 						4 ℃ for 6 months
			 		
					Recovery 						Matrices listed below were spiked with certain level of ALCAM and the recovery rates were calculated by comparing the measured value to the expected amount of ALCAM in samples.
																		Matrix 						Recovery range(%) 						Average(%) 					
											serum(n=5) 						85-105 						92 					
											EDTA plasma(n=5) 						92-99 						96 					
											heparin plasma(n=5) 						89-105 						99 					
				
			
			 		
					Linearity 						The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALCAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
																		Sample 						1:2 						1:4 						1:8 						1:16 					
											serum(n=5) 						87-105% 						86-105% 						86-103% 						89-105% 					
											EDTA plasma(n=5) 						82-98% 						85-101% 						87-98% 						82-97% 					
											heparin plasma(n=5) 						84-98% 						80-94% 						80-99% 						82-96% 					
				
			
			 		
					CV(%) 						Intra-Assay: CV<8%
			Inter-Assay: CV<10%
			 		
					Note 						For Research Use Only

Human ALCAM(CD166 antigen) ELISA Kit

Name: Human ALCAM(CD166 antigen) ELISA Kit
Brand: Medico
SKU: EB0001

SKU: EB0001

$440

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Principle of the Assay:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-
ALCAMantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ALCAM
antibody was used as detection antibodies. The standards, test samples and biotin conjugated
detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin
was added and unbound conjugates were washed away with wash buffer. TMB
substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to
produce a blue color product that changed into yellow after adding acidic stop solution. The
density of yellow is proportional to the ALCAM amount of sample captured in plate. Read the
O.D. absorbance at 450nm in a microplate reader, and then the concentration of ALCAM can be
calculated.
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Human ALCAM(CD166 antigen) ELISA Kit

Human ALCAM(CD166 antigen) ELISA Kit

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