Human ALCAM(CD166 antigen) ELISA Kit
			
			Product name						
Human ALCAM(CD166 antigen) ELISA Kit
							
		
			Catalogue No.						
EH0001
							
		
			Alias						
ALCAM ELISA Kit, CD166 ELISA Kit, MEMD ELISA Kit, activated leucocyte cell adhesion molecule ELISA Kit, CD166 antigen ELISA Kit
							
		
			Detection method						
Sandwich ELISA, Double Antibody
							
		
			Application						
ALCAM ELISA Kit allows for the in vitro quantitative determination of ALCAM concentrations in plasma, tissue homogenates and other biological fluids.
							
		
			Size						
96T
							
		
			Range						
62.5-4000pg/ml
							
		
			Sensitivity						
< 37.5pg/ml
							
		
			Storage						
4 ℃ for 6 months
							
		
			Recovery						
Matrices listed below were spiked with certain level of ALCAM and the recovery rates were calculated by comparing the measured value to the expected amount of ALCAM in samples.
			
									
						Matrix						Recovery range(%)						Average(%)					
					
						serum(n=5)						85-105						92					
					
						EDTA plasma(n=5)						92-99						96					
					
						heparin plasma(n=5)						89-105						99					
							
							
		
			Linearity						
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALCAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
			
									
						Sample						1:2						1:4						1:8						1:16					
					
						serum(n=5)						87-105%						86-105%						86-103%						89-105%					
					
						EDTA plasma(n=5)						82-98%						85-101%						87-98%						82-97%					
					
						heparin plasma(n=5)						84-98%						80-94%						80-99%						82-96%					
							
							
		
			CV(%)						
Intra-Assay: CV<8%
			Inter-Assay: CV<10%
							
		
			Note						
For Research Use Only

Human ALCAM(CD166 antigen) ELISA Kit

SKU: EB0001
$440Price

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    Principle of the Assay:

    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-
    ALCAMantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ALCAM
    antibody was used as detection antibodies. The standards, test samples and biotin conjugated
    detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin
    was added and unbound conjugates were washed away with wash buffer. TMB
    substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to
    produce a blue color product that changed into yellow after adding acidic stop solution. The
    density of yellow is proportional to the ALCAM amount of sample captured in plate. Read the
    O.D. absorbance at 450nm in a microplate reader, and then the concentration of ALCAM can be
    calculated.