Details
	
		
			
Description:
			
Bovine 25-OHD2(Vitamin D2) ELISA Kit
		
		
			
Applications:
			
cELISA
		
		
			
Specificity:
			
Bovine
		
		
			
Range:
			
1.563-100ng/ml
		
		
			
Sensitivity:
			
0.938ng/ml
		
	
 
	
Specifications
	
		
			
Product name
			
			
Bovine 25-OHD2(Vitamin D2) ELISA Kit
			
		
		
			
Catalogue No.
			
			
EB0072
			
		
		
			
Alias
			
			
25-OHD2 ELISA Kit
			
		
		
			
Detection method
			
			
Competitive ELISA, Coated with Antigen
			
		
		
			
Size
			
			
96T
			
		
		
			
Range
			
			
1.563-100ng/ml
			
		
		
			
Sensitivity
			
			
< 0.938ng/ml
			
		
		
			
Storage
			
			
4 ℃ for 6 months
			
		
		
			
Recovery
			
			
Matrices listed below were spiked with certain level of and the recovery rates were calculated by comparing the measured value to the expected amount of in samples.
			
				
					
						
Matrix
						
Recovery range(%)
						
Average(%)
					
					
						
serum(n=5)
						
92-97
						
95
					
					
						
EDTA plasma(n=5)
						
94-105
						
99
					
					
						
heparin plasma(n=5)
						
85-101
						
93
					
				
			
			
		
		
			
Linearity
			
			
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
			
				
					
						
Sample
						
1:2
						
1:4
						
1:8
						
1:16
					
					
						
serum(n=5)
						
86-96%
						
88-98%
						
85-104%
						
92-102%
					
					
						
EDTA plasma(n=5)
						
83-100%
						
82-101%
						
91-99%
						
87-101%
					
					
						
heparin plasma(n=5)
						
81-99%
						
80-96%
						
80-98%
						
86-100%
					
				
			
			
		
		
			
CV(%)
			
			
Intra-Assay: CV<8%
			Inter-Assay: CV<10%
			
		
		
			
Note
			
			
For Research Use Only